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简单介绍:
5-Lipoxygenase Activating Protein, ELISA Kit
详情介绍:
Product Name

5-Lipoxygenase Activating Protein (FLAP), ELISA Kit

Full Product Name

Rat 5-Lipoxygenase Activating Protein ELISA Kit

Product Synonym Names
FLAP
Product Gene Name

FLAP elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
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OMIM
601367
Species Reactivity
Rat
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FLAP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for FLAP purchase
MBS760576 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-Lipoxygenase Activating Protein (FLAP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FLAP. The ELISA analytical biochemical technique of the MBS760576 kit is based on FLAP antibody-FLAP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FLAP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FLAP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for
FLAP elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat FLAP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat FLAP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat FLAP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat FLAP can be calculated.

Typical Testing Data of FLAP elisa kit
FLAP elisa kit Typical Testing Data image
Typical Standard Curve of FLAP elisa kit
FLAP elisa kit Typical Standard Curve image
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NCBI/Uniprot data below describe general gene information for FLAP. It may not necessarily be applicable to this product.
NCBI GI #
182658
NCBI GeneID
241
NCBI Accession #
AAA35845.1 [Other Products]
UniProt Secondary Accession #
Q5VV04[Other Products]
UniProt Related Accession #
P20292[Other Products]
Molecular Weight
18,157 Da
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NCBI Official Full Name
5-lipoxygenase activating protein
NCBI Official Synonym Full Names
arachidonate 5-lipoxygenase activating protein
NCBI Official Symbol
ALOX5AP  [Similar Products]
NCBI Official Synonym Symbols
FLAP
  [Similar Products]
NCBI Protein Information
arachidonate 5-lipoxygenase-activating protein
UniProt Protein Name
Arachidonate 5-lipoxygenase-activating protein
UniProt Synonym Protein Names
FLAP; MK-886-binding protein
UniProt Gene Name
ALOX5AP  [Similar Products]
UniProt Synonym Gene Names
FLAP  [Similar Products]
UniProt Entry Name
AL5AP_HUMAN
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NCBI Summary for FLAP
This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]
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UniProt Comments for FLAP
ALOX5AP: Required for leukotriene biosynthesis by ALOX5 (5- lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition. Belongs to the MAPEG family.

Protein type: Activator; Membrane protein, integral; Nuclear envelope; Membrane protein, multi-pass; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; membrane; nuclear envelope; nuclear membrane

Molecular Function: arachidonic acid binding; enzyme activator activity; glutathione peroxidase activity; glutathione transferase activity; leukotriene-C4 synthase activity; protein binding; protein N-terminus binding

Biological Process: arachidonic acid metabolic process; leukotriene biosynthetic process; leukotriene metabolic process; lipoxygenase pathway; positive regulation of catalytic activity

Disease: Stroke, Ischemic
Research Articles on FLAP
1. Report no association between ALOX5AP SNPs and atherosclerotic plaque phenotypes.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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