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简单介绍:
A disintegrin and metalloproteinase with thrombospondin motifs 20, ELISA Kit
详情介绍:
Product Name

A disintegrin and metalloproteinase with thrombospondin motifs 20 (ADAMTS20), ELISA Kit

Full Product Name

Human A disintegrin and metalloproteinase with thrombospondin motifs 20 ELISA Kit

Product Synonym Names
A disintegrin and metalloproteinase with thrombospondin motifs 20; ADAM-TS 20; ADAM-TS20; ADAMTS-20; ADAMTS20
Product Gene Name

ADAMTS20 elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
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OMIM
611681
3D Structure
ModBase 3D Structure for P59510
Species Reactivity
Human
Specificity
This assay recognizes recombinant and natural human ADAM-TS 20. No significant cross-reactivity or interference was observed.
Samples
Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
Detection Range
78.0 pg/mL -5000 pg/mL.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ADAMTS20 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ADAMTS20 purchase
MBS2880879 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A disintegrin and metalloproteinase with thrombospondin motifs 20 (ADAMTS20) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAMTS20. The ELISA analytical biochemical technique of the MBS2880879 kit is based on ADAMTS20 antibody-ADAMTS20 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTS20 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTS20. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for
ADAMTS20 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human a disintegrin and metalloproteinase with thrombospondin motifs 20,ADAM-TS 20 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADAM-TS 20. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ADAM-TS 20 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ADAM-TS 20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADAM-TS 20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of ADAMTS20 elisa kit
ADAMTS20 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for ADAMTS20. It may not necessarily be applicable to this product.
NCBI GI #
124430557
NCBI GeneID
80070
NCBI Accession #
NP_079279.3 [Other Products]
NCBI GenBank Nucleotide #
NM_025003.3 [Other Products]
UniProt Primary Accession #
P59510 [Other Products]
UniProt Secondary Accession #
A6NNC9; J3QT00[Other Products]
UniProt Related Accession #
P59510[Other Products]
Molecular Weight
214,720 Da
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NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 20 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif 20
NCBI Official Symbol
ADAMTS20  [Similar Products]
NCBI Official Synonym Symbols
GON-1; ADAM-TS20; ADAMTS-20
  [Similar Products]
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 20
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 20
Protein Family
A disintegrin and metalloproteinase with thrombospondin motifs
UniProt Gene Name
ADAMTS20  [Similar Products]
UniProt Synonym Gene Names
ADAM-TS 20; ADAM-TS20; ADAMTS-20  [Similar Products]
UniProt Entry Name
ATS20_HUMAN
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NCBI Summary for ADAMTS20
The protein encoded by this gene is a member of the ADAMTS family of zinc-dependent proteases. The encoded protein has a signal peptide that is cleaved to release the mature peptide, which is secreted and found in the extracellular matrix. This protein may be involved in tissue remodeling. [provided by RefSeq, Sep 2011]
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UniProt Comments for ADAMTS20
ADAMTS20: May play a role in tissue-remodeling process occurring in both normal and pathological conditions. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.-; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Protease; Apoptosis

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix

Molecular Function: metalloendopeptidase activity; zinc ion binding

Biological Process: extracellular matrix organization and biogenesis; negative regulation of apoptosis; positive regulation of melanocyte differentiation; positive regulation of signal transduction; proteolysis
Research Articles on ADAMTS20
1. In summary, this study provides genetic evidence for a role of ADAMTS20 in CL/P development in dogs and as a candidate gene for CL/P development in humans.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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