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  • 产品名称:C5a anaphylatoxin chemotactic receptor, ELISA Kit

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简单介绍:
C5a anaphylatoxin chemotactic receptor, ELISA Kit
详情介绍:
Product Name

C5a anaphylatoxin chemotactic receptor (C5AR1), ELISA Kit

Full Product Name

Guinea pig C5a anaphylatoxin chemotactic receptor (C5AR1) ELISA Kit

Product Gene Name

C5AR1 elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
ModBase 3D Structure for O70129
Species Reactivity
Guinea pig
Specificity
This assay has high sensitivity and excellent specificity for detection of Guinea pig C5AR1. No significant cross-reactivity or interference between Guinea pig C5AR1 and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of C5AR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for C5AR1 purchase
MBS281243 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C5a anaphylatoxin chemotactic receptor (C5AR1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C5AR1. The ELISA analytical biochemical technique of the MBS281243 kit is based on C5AR1 antibody-C5AR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C5AR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C5AR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for
C5AR1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate C5AR1 in samples. An antibody specific for C5AR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyC5AR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C5AR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C5AR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for C5AR1. It may not necessarily be applicable to this product.
NCBI GI #
290543382
NCBI GeneID
100135489
NCBI Accession #
NP_001166393.1 [Other Products]
NCBI GenBank Nucleotide #
NM_001172922.1 [Other Products]
UniProt Primary Accession #
O70129 [Other Products]
UniProt Related Accession #
O70129[Other Products]
Molecular Weight
38,592 Da
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NCBI Official Full Name
C5a anaphylatoxin chemotactic receptor 1
NCBI Official Symbol
C5ar1  [Similar Products]
NCBI Official Synonym Symbols
C5R1
  [Similar Products]
NCBI Protein Information
C5a anaphylatoxin chemotactic receptor 1
UniProt Protein Name
C5a anaphylatoxin chemotactic receptor 1
UniProt Synonym Protein Names
C5a anaphylatoxin chemotactic receptor; C5a-R; C5aR; CD_antigen: CD88
Protein Family
C5a anaphylatoxin chemotactic receptor
UniProt Gene Name
C5AR1  [Similar Products]
UniProt Synonym Gene Names
C5R1; C5a-R; C5aR  [Similar Products]
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UniProt Comments for C5AR1
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a (PubMed:9576615). The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events (). Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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