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简单介绍:
C5a anaphylatoxin chemotactic receptor (C5AR1), ELISA Kit
详情介绍:
Product Name

C5a anaphylatoxin chemotactic receptor (C5AR1), ELISA Kit

Full Product Name

Sheep C5a anaphylatoxin chemotactic receptor (C5AR1) ELISA Kit

Product Gene Name

C5AR1 elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sample Manual Insert
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OMIM
113995
Species Reactivity
Sheep
Specificity
This assay has high sensitivity and excellent specificity for detection of C5AR1. No significant cross-reactivity or interference between C5AR1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C5AR1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of C5AR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for C5AR1 purchase
MBS7205720 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C5a anaphylatoxin chemotactic receptor (C5AR1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C5AR1. The ELISA analytical biochemical technique of the MBS7205720 kit is based on C5AR1 antibody-C5AR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C5AR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C5AR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for
C5AR1 elisa kit
Intended Uses: This C5AR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep C5AR1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||C5AR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-C5AR1 antibody and an C5AR1-HRP conjugate. The assay sample and buffer are incubated together with C5AR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C5AR1 concentration since C5AR1 from samples and C5AR1-HRP conjugate compete for the anti-C5AR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by C5AR1 from the sample, fewer sites are left to bind C5AR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C5AR1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for C5AR1 elisa kit
Signal Transduction

Typical Testing Data/Standard Curve of C5AR1 elisa kit
C5AR1 elisa kit Typical Testing Data/Standard Curve image
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NCBI/Uniprot data below describe general gene information for C5AR1. It may not necessarily be applicable to this product.
NCBI GI #
311033355
NCBI GeneID
728
NCBI Accession #
P21730.2 [Other Products]
UniProt Related Accession #
P21730[Other Products]
Molecular Weight
39,336 Da
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NCBI Official Full Name
C5a anaphylatoxin chemotactic receptor 1
NCBI Official Synonym Full Names
complement component 5a receptor 1
NCBI Official Symbol
C5AR1  [Similar Products]
NCBI Official Synonym Symbols
C5A; C5AR; C5R1; CD88
  [Similar Products]
NCBI Protein Information
C5a anaphylatoxin chemotactic receptor 1; C5a-R; C5a ligand; C5a anaphylatoxin receptor; complement component 5 receptor 1
UniProt Protein Name
C5a anaphylatoxin chemotactic receptor 1
UniProt Synonym Protein Names
C5a anaphylatoxin chemotactic receptor; C5a-R; C5aR; CD_antigen: CD88
Protein Family
C5a anaphylatoxin chemotactic receptor
UniProt Gene Name
C5AR1  [Similar Products]
UniProt Synonym Gene Names
C5AR; C5R1; C5a-R; C5aR  [Similar Products]
UniProt Entry Name
C5AR1_HUMAN
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UniProt Comments for C5AR1
C5aR: a family 1 G-protein coupled receptor that stimulates the GTPase activity of Gi2. Receptor for the chemotactic and inflammatory complement factor C5a. Stimulates chemotaxis, granule enzyme release and superoxide anion production. Phosphorylated in response to C5a or after stimulation of cells with phorbol esters. Expressed on monocytes, granulocytes, dendritic cells, astrocytes and microglia.

Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: cell surface; basolateral plasma membrane; integral to plasma membrane; apical part of cell; cytoplasmic membrane-bound vesicle; plasma membrane

Molecular Function: complement component C5a binding; complement component C5a receptor activity; C5a anaphylatoxin receptor activity

Biological Process: neutrophil chemotaxis; organ regeneration; sensory perception of chemical stimulus; apoptosis; activation of MAPK activity; response to lipopolysaccharide; signal transduction; chemotaxis; cell proliferation in hindbrain; mRNA transcription from RNA polymerase II promoter; elevation of cytosolic calcium ion concentration; defense response to Gram-positive bacterium; phospholipase C activation; response to peptidoglycan; cellular defense response; immune response; negative regulation of neuron apoptosis; inflammatory response; positive regulation of epithelial cell proliferation
Research Articles on C5AR1
1. High levels of CD88 are associated with poor prognosis of NSCLC.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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