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  • 产品名称:17-keto-steroid (17-KS), ELISA Kit

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简单介绍:
17-keto-steroid (17-KS), ELISA Kit
详情介绍:
Product Name

17-keto-steroid (17-KS), ELISA Kit

Full Product Name

Human 17-keto-steroid (17-KS) ELISA Kit

Product Gene Name

17-KS elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
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Species Reactivity
Human
Samples
Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant 17-KS concentration
Assay Type
Sandwich
Detection Range
100 umol/L-1.56 umol/L
Sensitivity
0.5umol/L.
Intra-***** Precision
<= 8%<="" div="">
Inter-***** Precision
<= 12%<="" div="">
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit *****s are dynamic research tools and sometimes they may be updated and improved. If the format of this ***** is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the *****. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of 17-KS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for 17-KS purchase
MBS262237 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent *****) Kit for analyzing the presence of the 17-keto-steroid (17-KS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 17-KS. The ELISA analytical biochemical technique of the MBS262237 kit is based on 17-KS antibody-17-KS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 17-KS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 17-KS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control *****s assessing reproducibility identified the intra-***** CV (%) and inter-***** CV(%).
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Related Product Information for
17-KS elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human 17-KS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of 17-KS elisa kit
17-KS elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for 17-KS. It may not necessarily be applicable to this product.
NCBI GI #
1513310
NCBI GeneID
15487
NCBI Accession #
AAB06793.1 [Other Products]
UniProt Secondary Accession #
G3UWF9[Other Products]
UniProt Related Accession #
P70385[Other Products]
Molecular Weight
34,330 Da
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NCBI Official Full Name
17-beta-hydroxysteroid dehydrogenase type 3
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 3
NCBI Official Symbol
Hsd17b3  [Similar Products]
NCBI Protein Information
testosterone 17-beta-dehydrogenase 3; 17-beta-HSD 3; 17(beta)HSD type 3; 17-beta-hydroxysteroid dehydrogenase type 3; testicular 17-beta-hydroxysteroid dehydrogenase
UniProt Protein Name
Testosterone 17-beta-dehydrogenase 3
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase type 3; 17-beta-HSD 3; Testicular 17-beta-hydroxysteroid dehydrogenase
UniProt Gene Name
Hsd17b3  [Similar Products]
UniProt Synonym Gene Names
Edh17b3; 17-beta-HSD 3  [Similar Products]
UniProt Entry Name
DHB3_MOUSE
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UniProt Comments for 17-KS
HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: Lipid Metabolism - androgen and estrogen; EC 1.1.1.64; Oxidoreductase

Cellular Component: intracellular membrane-bound organelle

Molecular Function: estradiol 17-beta-dehydrogenase activity; testosterone 17-beta-dehydrogenase (NADP+) activity; oxidoreductase activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity

Biological Process: metabolic process; lipid metabolic process; steroid biosynthetic process
Research Articles on 17-KS
1. Enzyme activities of 3-betaHSDH increase as the sound pressure levels increase in adrenal cortex zona fasciculate polygonal cells.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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